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rhigf 1r protein  (R&D Systems)


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    Structured Review

    R&D Systems rhigf 1r protein
    Rhigf 1r Protein, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+igf+1r/Recombinant+Human+IGF-I+R%2FIGF1R+Protein%2C+CF/us11918656-638-16-18
    Average 94 stars, based on 33 article reviews
    rhigf 1r protein - by Bioz Stars, 2026-09
    94/100 stars

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    Recombinant:

    Article Title: Anti-IGR-1R antibodies and uses thereof
    Article Snippet: A biotin-labeled anti-HisTag antibody (biotin-PENTA-His, Qiagen Cat. No. 34440) was immobilized to saturation on a Biacore SA chip (Cat. No. BR-1000-32) surface by injection at 500 nM in HBS-EP buffer. .. Recombinant human IGF-1R-10His (R&D Systems, Cat. No. 305-GR-050) was captured on the biotin-PENTA-His surface by injecting 20 μL of 40 nM protein at 2 μL/min. ..

    Article Title: IGF-1R binding polypeptides and their use
    Article Snippet: .. Biotinylation of target protein: Recombinant human IGF-1R(R&D Systems #391-GR) in PBS was biotinylated with a 10× molar excess of Sulfo-NHS-LC-Biotin (Pierce #21327) under 30 min incubation at room temperature. .. In order to remove any excess biotin, a buffer exchange was made on spin columns (Pierce #89849) pre-equilibrated with PBS according to the manufacturer's instructions.

    Article Title: Evaluation of a novel hexavalent humanized anti-IGF-1R antibody and its bivalent parental IgG in diverse cancer cell lines.
    Article Snippet: Humanized antibodies, including hR1, hPAM4 (anti-mucin), hA20 (anti-CD20), hRS7 (anti-Trop-2), hLL2 (anti-CD22), and hMN-15 (anti-CEACAM6), were provided by Immunomedics. .. Recombinant human IGF-1R (rhIGF-1R), recombinant human IGF-1, recombinant human IGF-2, and mouse anti-human IGF-1R mAb (MAB391) were obtained from R&D Systems. .. Additional anti-human IGF-1R antibodies were obtained from Calbiochem for Ab-1 (clone aIR3), Ab-3 (Clone 33255.111), and Ab-4 (clone 24–31); from Santa Cruz Biotechnology for 2C8, 1H7, 3B7, and C-20; from Millipore for 24–57 and 24–31; and from Invitrogen for 17–69, 24–60, and 1–2.

    Article Title: Insulin and IGF-1 receptor agonists and antagonists
    Article Snippet: To quantitate cell number, 10 μl of WST-1 reagent (Roche Molecular Biochemicals, Indianapolis, Ind.) was added to each well and the plates were returned to the 37° C./CO2 incubator for approximately 2 h. Measurements were then read at 440 nm, with 700 nm used as a reference. .. E. Binding (ALPHAScreen) Assays To assay binding, the relative potencies of peptides as compared to IGF-1 were analyzed in a competition system utilizing biotinylated-human IGF-1 (b-hIGF-1) and His-tagged soluble recombinant human IGF-1R (srhIGF-1R-his; R&D systems, Inc., Minneapolis, Minn.). .. Detection of the receptor ligand interaction was measured in an amplified luminescent proximity homogeneous assay (ALPHAScreen; BioSignal-Packard, Montreal).

    Article Title: Anti-IGF antibodies
    Article Snippet: Bound IGF-1 or IGF-2 is detected using alkaline phosphatase-conjugated streptavidin (1:10,000 dilution) (Roche). .. Selection of Antibodies that Neutralize IGF-1 and IGF-2 Binding to IGF-1R To identify IGF antibodies that neutralize the ability of IGF-1 and IGF-2 to bind to the IGF-1R, an ELISA is established by coating plates with 1.5 μg/ml recombinant human IGF-1R (R&D Systems) which allows the binding of biotinylated IGF-1 and biotinylated IGF-2. .. Bound IGF-1 or IGF-2 is detected using alkaline phosphatase-conjugated streptavidin (1:10,000 dilution) (Roche).

    Article Title: Anti-IGF-1R antibodies and uses thereof
    Article Snippet: A biotin-labeled anti-HisTag antibody (biotin-PENTA-His, Qiagen Cat. No. 34440) was immobilized to saturation on a Biacore SA chip (Cat. No. BR-1000-32) surface by injection at 500 nM in HBS-EP buffer. .. Recombinant human IGF-1R-10His (R&D Systems, Cat. No. 305-GR-050) was captured on the biotin-PENTA-His surface by injecting 20 μL of 40 nM protein at 2 μL/min. ..

    Incubation:

    Article Title: IGF-1R binding polypeptides and their use
    Article Snippet: .. Biotinylation of target protein: Recombinant human IGF-1R(R&D Systems #391-GR) in PBS was biotinylated with a 10× molar excess of Sulfo-NHS-LC-Biotin (Pierce #21327) under 30 min incubation at room temperature. .. In order to remove any excess biotin, a buffer exchange was made on spin columns (Pierce #89849) pre-equilibrated with PBS according to the manufacturer's instructions.

    Binding Assay:

    Article Title: Insulin and IGF-1 receptor agonists and antagonists
    Article Snippet: To quantitate cell number, 10 μl of WST-1 reagent (Roche Molecular Biochemicals, Indianapolis, Ind.) was added to each well and the plates were returned to the 37° C./CO2 incubator for approximately 2 h. Measurements were then read at 440 nm, with 700 nm used as a reference. .. E. Binding (ALPHAScreen) Assays To assay binding, the relative potencies of peptides as compared to IGF-1 were analyzed in a competition system utilizing biotinylated-human IGF-1 (b-hIGF-1) and His-tagged soluble recombinant human IGF-1R (srhIGF-1R-his; R&D systems, Inc., Minneapolis, Minn.). .. Detection of the receptor ligand interaction was measured in an amplified luminescent proximity homogeneous assay (ALPHAScreen; BioSignal-Packard, Montreal).

    Article Title: Anti-IGF antibodies
    Article Snippet: Bound IGF-1 or IGF-2 is detected using alkaline phosphatase-conjugated streptavidin (1:10,000 dilution) (Roche). .. Selection of Antibodies that Neutralize IGF-1 and IGF-2 Binding to IGF-1R To identify IGF antibodies that neutralize the ability of IGF-1 and IGF-2 to bind to the IGF-1R, an ELISA is established by coating plates with 1.5 μg/ml recombinant human IGF-1R (R&D Systems) which allows the binding of biotinylated IGF-1 and biotinylated IGF-2. .. Bound IGF-1 or IGF-2 is detected using alkaline phosphatase-conjugated streptavidin (1:10,000 dilution) (Roche).

    Amplified Luminescent Proximity Homogenous Assay:

    Article Title: Insulin and IGF-1 receptor agonists and antagonists
    Article Snippet: To quantitate cell number, 10 μl of WST-1 reagent (Roche Molecular Biochemicals, Indianapolis, Ind.) was added to each well and the plates were returned to the 37° C./CO2 incubator for approximately 2 h. Measurements were then read at 440 nm, with 700 nm used as a reference. .. E. Binding (ALPHAScreen) Assays To assay binding, the relative potencies of peptides as compared to IGF-1 were analyzed in a competition system utilizing biotinylated-human IGF-1 (b-hIGF-1) and His-tagged soluble recombinant human IGF-1R (srhIGF-1R-his; R&D systems, Inc., Minneapolis, Minn.). .. Detection of the receptor ligand interaction was measured in an amplified luminescent proximity homogeneous assay (ALPHAScreen; BioSignal-Packard, Montreal).

    Selection:

    Article Title: Anti-IGF antibodies
    Article Snippet: Bound IGF-1 or IGF-2 is detected using alkaline phosphatase-conjugated streptavidin (1:10,000 dilution) (Roche). .. Selection of Antibodies that Neutralize IGF-1 and IGF-2 Binding to IGF-1R To identify IGF antibodies that neutralize the ability of IGF-1 and IGF-2 to bind to the IGF-1R, an ELISA is established by coating plates with 1.5 μg/ml recombinant human IGF-1R (R&D Systems) which allows the binding of biotinylated IGF-1 and biotinylated IGF-2. .. Bound IGF-1 or IGF-2 is detected using alkaline phosphatase-conjugated streptavidin (1:10,000 dilution) (Roche).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Anti-IGF antibodies
    Article Snippet: Bound IGF-1 or IGF-2 is detected using alkaline phosphatase-conjugated streptavidin (1:10,000 dilution) (Roche). .. Selection of Antibodies that Neutralize IGF-1 and IGF-2 Binding to IGF-1R To identify IGF antibodies that neutralize the ability of IGF-1 and IGF-2 to bind to the IGF-1R, an ELISA is established by coating plates with 1.5 μg/ml recombinant human IGF-1R (R&D Systems) which allows the binding of biotinylated IGF-1 and biotinylated IGF-2. .. Bound IGF-1 or IGF-2 is detected using alkaline phosphatase-conjugated streptavidin (1:10,000 dilution) (Roche).



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